Observing the Reversible Single Molecule Electrochemistry of Alexa Fluor 647 Dyes by Total Internal Reflection Fluorescence Microscopy
نویسندگان
چکیده
منابع مشابه
* Single - molecule fluorescence imaging by total internal reflection fluorescence microscopy ( IUPAC Technical Report )
Total internal reflection fluorescence (TIRF) is a popular illumination technique in microscopy, with many applications in cell and molecular biology and biophysics. The chief advantage of the technique is the high contrast that can be achieved by restricting fluorescent excitation to a thin layer. We summarise the optical theory needed to understand the technique and various aspects required f...
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ژورنال
عنوان ژورنال: Angewandte Chemie
سال: 2019
ISSN: 0044-8249,1521-3757
DOI: 10.1002/ange.201907298